Characterization of bovine serum albumin/ chlorogenic acid solution mixtures by analytical ultracentrifugation
نویسنده
چکیده
Chlorogenic acid (CGA) is widely distributed in the plant kingdom.For example, green coffee beans contain 6–10% CGA, and a 200-ml cup of coffee may have 20–675 mg CGA, depending on the roasting and brewing conditions [1]. In potato tubers CGA constitutes up to 90% of the total phenolic content [2]. CGAplays an important role in Progr Colloid Polym Sci (2004) 127: 83–88 DOI 10.1007/b98017 Springer-Verlag 2004
منابع مشابه
The Structure and Nature of Protein-Polvsaccharide Complexes
Many food systems consist of mixtures of protein and polysaccharide and many are also thermally processed. Potential interaction phenomena in solutions ofprotein and polysaccharide are described using the following probes: sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation, dynamic light scattering and total intensity light scattering coupled to an on-line gel p...
متن کاملThe correlation of plasma proteins binding capacity and flavopiridol cellular and clinical trial studies
Previous clinical research has suggested high-affinity binding of flavopiridol (FLAP) to human blood serum proteins, specifically either human serum albumin (HSA) or human alpha-1-acid glycoprotein (hAGP), when compared to fetal bovine serum albumin (BSA) or bovine alpha-1-acid glycoprotein (bAGP) used in pre-clinical assays. This high-affinity binding was suggested as the reason for its poor h...
متن کاملA centrifugation-based physicochemical characterization method for the interaction between proteins and nanoparticles
Nanomedicine requires in-depth knowledge of nanoparticle-protein interactions. These interactions are studied with methods limited to large or fluorescently labelled nanoparticles as they rely on scattering or fluorescence-correlation signals. Here, we have developed a method based on analytical ultracentrifugation (AUC) as an absorbance-based, label-free tool to determine dissociation constant...
متن کامل3D-Printing for Analytical Ultracentrifugation
Analytical ultracentrifugation (AUC) is a classical technique of physical biochemistry providing information on size, shape, and interactions of macromolecules from the analysis of their migration in centrifugal fields while free in solution. A key mechanical element in AUC is the centerpiece, a component of the sample cell assembly that is mounted between the optical windows to allow imaging a...
متن کاملCharacterization of hydroxycinnamic acid derivatives binding to bovine serum albumin.
Hydroxycinnamic acid derivatives (HCAs) are a group of naturally occurring polyphenolic compounds which possess various pharmacological activities. In this work, the interactions of bovine serum albumin (BSA) with six HCA derivatives, including chlorogenic acid (CHA), caffeic acid (CFA), m-coumaric acid (m-CA), p-coumaric acid (p-CA), ferulic acid (FA) and sinapic acid (SA) have been investigat...
متن کامل